Naïve T cell activation in the draining lymph nodes is accelerated by airway resident CD4 T cell activation

Abstract Tissue-resident memory CD4 T cells (TRM) protect against pathogens through the early shaping of inflammation at sites infection. The extent to which their activation can impact subsequent systemic cell responses is not clear. To ask if lung TRM recognizing antigen same presented in secondary lymphoid organs, we generated OVA-specific primed by infection with an influenza virus expressing OVA peptide. We isolated 30 days after and transferred one million naive mice intranasally resulting airway-resident cohort similar magnitude virus-primed mice. then recalled intranasal administration FITC-labelled protein focus on recall absence virus-induced inflammation. One day challenge more FITC+ APC were present draining lymph nodes (dLN) TRM, suggesting presentation may be accelerated presence airway TRM. confirmed this tracking (OT-II) CD8 (OT-I) cohorts i.v. harboring or not: responding cells, higher levels markers, detected 3 By 4 days, lungs recipients, indicating that form a circuit via enhanced nodes. Our results suggest kinetics induced airways represent novel mechanism protection other respiratory viruses.